Effects of Sec-O-Glucosylhamaudol on expressions of nitric oxide and interleukin-6 in Lipopolysaccharide stimulated murine macrophage
10.3969/j.issn.1000-484X.2015.11.009
- VernacularTitle:亥茅酚苷对脂多糖诱导巨噬细胞一氧化氮和白介素6表达的影响
- Author:
Chao LUO
;
Li SHEN
- Publication Type:Journal Article
- Keywords:
Sec-O-Glucosylhamaudol;
Macrophage;
Inflammation;
Nitric oxide;
Interleukin-6
- From:
Chinese Journal of Immunology
2015;(11):1486-1489
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Sec-O-Glucosylhamaudol on inflammatory mediator and cytokine( NO and IL-6)production in Lipopolysaccharide(LPS) stimulated murine macrophage(RAW264.7).Methods: Macrophages were induced with LPS,and incubated with different concentrations of Sec-O-Glucosylhamaudol(20,40,80 μg/ml),the quantity of NO production was measured by Griess reagent;the IL-6 production were measured by enzyme linked immunosorbent assay(ELISA),the expression of nitric oxide synthase( iNOS) in cells were detected by Western blot;the expression of iNOS and IL-6 mRNA were analyzed by real-time PCR.Results:Each concentrations of Sec-O-Glucosylhamaudol(20,40,80 μg/ml) inhibited the production of NO and IL-6 in LPS-stimulated RAW264.7 cells(P<0.01).This compound also reduced the mRNA expression of iNOS and IL-6.Conclusion:Sec-O-Glu-cosylhamaudol exhibited anti-inflammatory activity by inhibited the NO and IL-6 production in LPS stimulated RAW264.7 cells.
