Inhibition of FoxM1 sensitizes leukemia K562 cells to homoharringtonine
10.3969/j.issn.1000-4718.2015.11.002
- VernacularTitle:抑制白血病 K562细胞 FoxM1表达可增强细胞对高三尖杉酯碱的敏感性
- Author:
Jin CHEN
;
Minran ZHOU
;
Ting SUN
;
Xuemei QIN
;
Zhongmin CHEN
;
Chunyan CHEN
;
Yuan YU
- Publication Type:Journal Article
- Keywords:
Homoharringtonine;
Forkhead box protein M1;
K562 cells;
Drug sensitivity
- From:
Chinese Journal of Pathophysiology
2015;(11):1928-1932
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study whether inhibition of forkhead box protein M1(FoxM1) sensitizes leukemia K562 cells to homoharringtonine ( HHT ) .METHODS: K562 cells were incubated with HHT at different concentrations ( 0μmol/L, 0.015 μmol/L, 0.030μmol/L and 0.045μmol/L) for different time (0 h, 24 h, 48 h and 72 h).The mRNA and protein levels of FoxM1 were detected by real-time PCR and Western blot.FoxM1 siRNA was transfected into K562 cells with 0.015μmol/L HHT after 6 h.After 72 h incubation, the cell proliferation was detected by cell counting and soft agar assay, and the proportion of apoptotic K562 cells was determined by flow cytometry.The expression of c-Myc and Sp1 were detected by real-time PCR and Western blot.RESULTS:FoxM1 expression was reduced time-dependently and dose-dependently, suggesting that HHT mediated the downregulation of FoxM1 in K562 cells.In K562 cells, treatment with FoxM1 siRNA and HHT inhibited the cell proliferation and promoted the apoptosis significantly.Therefore, inhibition of FoxM1 sensitized leukemia K562 cells to HHT.The expression of c-Myc and Sp1 was positively regulated by FoxM1. CONCLUSION:HHT inhibits Forkhead box protein M1 expression in K562 cells.Inhibition of FoxM1 sensitizes K562 cells to HHT.
