ARHI gene inhibits cell growth, induces G2/M phase arrest and apoptosis of acute myeloid leukemia cell line U937
10.3969/j.issn.1000-4718.2015.11.005
- VernacularTitle:ARHI基因抑制U937白血病细胞株生长并诱导其G2/M期阻滞及凋亡
- Author:
Ying LU
;
Xiangfu LIU
;
Lingling LIU
;
Fang LI
;
Xueling QIN
;
Dongjun LIN
- Publication Type:Journal Article
- Keywords:
ARHI gene;
Acute myeloid leukemia;
Cell cycle;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2015;(11):1950-1955
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the expression of aplasia rashomolog member I ( ARHI) gene in acute myeloid leukemia cells (AML) and to study the effects of ARHI on the growth of AML cell line U937.METHODS:The mRNA ex-pression of ARHI in AML cells, 293FT cells, AML primary cells and healthy volunteer blood cells were detected by RT-PCR.After transfection with the MSCV-IRES-GFP-ARHI plasmid to the U937 cells, the growth curve was analyzed by MTT assay.U937 cells were re-suspended by fresh medium and cultured for 24 h, then the cell cycle distribution and ap-optotic rate were determined.RESULTS:The mRNA of ARHI was positively detectable in 293FT cells and healthy volun-teer blood cells instead of AML cell line and AML primary cells.The growth curve showed that cell viability in U937 cells with high expression of ARHI (U937-ARHI) was lower than that in the control cells (U937-GFP) on 6th~8th day.The ratio of G2/M phase and apoptotic rate in the U937-ARHI cells were increased compare with control group ( P<0.05 ) . CONCLUSION:The mRNA level of ARHI is low in AML cells.High expression of ARHI gene in U937 cells inhibits cell growth, arrests the cells at G2/M phase and induces apoptosis.
