Cx43 is involved in electrical remodeling of atrial myocytes through regu-lating L-type calcium current
10.3969/j.issn.1000-4718.2015.11.010
- VernacularTitle:缝隙连接蛋白43通过调控L型钙电流参与心房肌细胞的电重塑
- Author:
Fang RAO
;
Yumei XUE
;
Chunyu DENG
;
Xiyong YU
;
Dingzhang XIAO
;
Shaoxian CHEN
;
Qiuxiong LIN
;
Hui YANG
;
Sujuan KUANG
;
Xiaoying LIU
;
Jiening ZHU
;
Shulin WU
- Publication Type:Journal Article
- Keywords:
Connexin 43;
L-type calcium channels;
Atrial myocytes;
Atrial fibrillation;
Electrical remodeling
- From:
Chinese Journal of Pathophysiology
2015;(11):1986-1991
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate whether the association of connexin 43 ( Cx43 ) and L-type calcium channel involved in the pathogenesis of atrial fibrillation ( AF) .METHODS:The biochemical assays and whole-cell patch-clamp technique were used to study the expression of Cx43 in human atrial tissue.The co-localization of Cx43 and L-type calcium channel, and the regulation of L-type calcium current in atrial myocytes were investigated.RESULTS:The expression of Cx43 at mRNA and protein levels was decreased in human atrial tissues of AF patients.In cultured atrium-derived myocytes ( HL-1 cells) , knockdown of Cx43 significantly inhibited the mRNA expression of L-type calcium channelα1c subunit, as well as L-type calcium current.Co-localization of Cx43 with L-type calcium channel α1c subunit in mouse atrial myocytes was observed.CONCLUSION:The decrease in Cx43 is involved in the pathogenesis of AF, probably through reducing the L-type calcium current in atrial myoctyes by co-localization with L-type calcium channel, thus representing the potential pathogenesis in atrial fibrillation.
