miR-21 inhibits apoptosis of Schwann cells following peripheral nerve in-jury
10.3969/j.issn.1000-4718.2015.11.020
- VernacularTitle:m iR－21在周围神经损伤时调控雪旺细胞凋亡
- Author:
Xinhua LU
;
Hui WANG
;
Xinjie NING
;
Juncheng LUO
;
Jiaji LIANG
- Publication Type:Journal Article
- Keywords:
Peripheral nerve injury;
MicroRNA-21;
Schwann cells;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2015;(11):2047-2052
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the relationship and molecular mechanism between microRNA-21(miR-21) and Schwann cells ( SC) following peripheral nerve injury.METHODS: The mRNA expression of miR-21 and phosphatase and tensin homologue deleted on chromosome ten ( PTEN) in animal model were detected by real-time PCR.The over-ex-pression of miR-21 and inhibition of miR-21 expression in the Schwann cells according to transfection of lentiviral vectors were performed, the nonspecific miRNA was used as a negative control ( NC) .The cell apoptosis was measured by flow cy-tometry.The mRNA expression of miR-21 and PTEN in the cells was detected by real-time PCR.The protein expression of PTEN and cleaved caspase-3 was determined by Western blot.RESULTS: The level of miR-21 was significantly higher and the mRNA level of PTEN was significantly lower in the model of nerve injury than those in control group.miR-21 over-expression decreased the number of apoptotic Schwann cells compared with NC-SC.The mRNA expression of PTEN was down-regulated by over-expression of miR-21.The protein expression of PTEN and cleaved caspase-3 was down-regulated by over-expression of miR-21 (P<0.05).CONCLUSION: miR-21 may play an important role in the peripheral nerve injury through inhibiting apoptosis of Schwann cells by down-regulating the expression of PTEN.
