Pro-inflammatory Cytokine Expression of Spleen Dendritic Cells in Mouse Toxoplasmosis.
10.3347/kjp.2011.49.2.109
- Author:
Ho Woo NAM
1
;
Hye Jin AHN
;
Hyun Jong YANG
Author Information
1. Department of Parasitology, College of Medicine, Catholic University of Korea, Seoul 137-701, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Toxoplasma gondii;
spleen;
dendritic cell;
cytokine;
FACS;
mouse
- MeSH:
Animals;
Antigens, CD11c/analysis;
Antigens, CD8/analysis;
Cytokines/*blood/*secretion;
Dendritic Cells/chemistry/*immunology;
Disease Models, Animal;
Flow Cytometry;
Mice;
Mice, Inbred BALB C;
Rodent Diseases/immunology;
Spleen/*immunology;
Time Factors;
Toxoplasmosis, Animal/*immunology
- From:The Korean Journal of Parasitology
2011;49(2):109-114
- CountryRepublic of Korea
- Language:English
-
Abstract:
Dendritic cells have been known as a member of strong innate immune cells against infectious organelles. In this study, we evaluated the cytokine expression of splenic dendritic cells in chronic mouse toxoplasmosis by tissue cyst-forming Me49 strain and demonstrated the distribution of lymphoid dendritic cells by fluorescence-activated cell sorter (FACS). Pro-inflammatory cytokines, such as IL-1alpha, IL-1beta, IL-6, and IL-10 increased rapidly at week 1 post-infection (PI) and peaked at week 3 PI. Serum IL-10 level followed the similar patterns. FACS analysis showed that the number of CD8alpha+/CD11c+ splenic dendritic cells increased at week 1 and peaked at week 3 PI. In conclusion, mouse splenic dendritic cells showed early and rapid cytokine changes and may have important protective roles in early phases of murine toxoplasmosis.
