Role of post-hemorrhagic shock mesenteric lymph drainage in restoring balance of ACE/ACE2 in kidney of mice
10.3969/j.issn.1000-4718.2015.11.028
- VernacularTitle:失血性休克后肠淋巴液引流恢复小鼠肾组织ACE／ACE2平衡的作用
- Author:
Junfen LIU
;
Lina JIANG
;
Limin ZHANG
;
Guiqing LIU
;
Zigang ZHAO
;
Shengjun LIU
;
Chunyu NIU
- Publication Type:Journal Article
- Keywords:
Hemorrhagic shock;
Lymph fluid;
Drainage;
Acute kidney injury;
Angiotensin-converting en-zyme
- From:
Chinese Journal of Pathophysiology
2015;(11):2096-2100
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the role of post-hemorrhagic shock mesenteric lymph (PHSML) drainage on the balance of angiotensin-converting enzyme ( ACE) and ACE2 in the kidney.METHODS:A hemorrhagic shock model was established and then fluid resuscitation was performed to the animals in shock and shock+drainage groups, and the PHMSL was drained in shock+drainage group after fluid resuscitation.After 6 h of resuscitation, the mRNA expression of ACE, ACE2, angiotensin II (Ang II) type 1 receptor (AT1R) and Mas-related G-protein-coupled receptor (MasR), and the levels of Ang II and Ang (1-7) in the renal tissues were observed.RESULTS:Hemorrhagic shock increased the levels of ACE mRNA, AT1R mRNA and Ang II, and decreased the levels of ACE2 mRNA, MasR mRNA and Ang(1-7) in the kid-ney.PHSML drainage abolished the effect of hemorrhagic shock on ACE2 and AT1R mRNA expression.Meanwhile, PHSML drainage reduced the hemorrhagic shock-induced increases in the ratios of ACE/ACE2, Ang II/Ang(1-7) and AT1R/MasR.CONCLUSION:The PHSML drainage restores the balance of ACE/ACE2, which is beneficial to alleviate acute kidney injury following hemorrhagic shock in the mice.
