Preparation of a deactivation vaccine against Aeromonas hydrophila and research about its immue effect in the north of Henan
10.3969/j.issn.1000-484X.2015.11.013
- VernacularTitle:豫北地区嗜水气单胞菌灭活疫苗的制备及免疫效果的研究
- Author:
Jianyi GUAN
;
Huili MAO
;
Wenxu HE
;
Limin YANG
;
Yundong FU
;
Jingmei ZHANG
;
Jiajing WU
;
Qianji NING
- Publication Type:Journal Article
- Keywords:
Aeromonas hydrophia;
Vaccine;
Antibody titers;
Tissue slices;
Immunization protection rate
- From:
Chinese Journal of Immunology
2015;(11):1501-1504
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Selected virulence factors more than and high virulent Aeromonas hydrophia strain made into inactivated vaccine,to study the immunization effect of inactivated vaccine.Methods:Crucian were vaccinated with formalin-killed vaccine via in-traperitoneal injection.Controls were injected with the same volumes of saline.Then the antibody titres, histopathology and relative percent survival were analyzed from samples of both groups.Results: The antibody in the indirect agglutination reaction could be detected in vaccinated fish once a weeks after immunization and reached highest level 6 weeks after immunization.The histopathology analysis indicated that the vaccine had a good protective effect on crucian target organs.Vaccinated fish showed 100%relative percent survival and the immune period would be 6 month.Conclusion:The vaccine in this study has a significant protective effect on crucian and may be used as effective fish vaccines against bacterial septicemia.