Human cartilage glycoprotein-39 induces chondrogenesis of precartilaginous stem cells
10.3969/j.issn.2095-4344.2015.42.013
- VernacularTitle:人类软骨糖蛋白39诱导关节软骨祖母细胞的成软骨分化
- Author:
Yueping CHEN
;
Panfeng DONG
;
Zhenzhong YUAN
;
Yi RAO
;
Jinhuan LI
;
Jie KANG
;
Xiaoyun ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(42):6793-6797
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Studies have shown that human cartilage glycoprotein-39 has a certain relationship to articular cartilage degeneration and repair, but the mechanism of action is not very clear. OBJECTIVE:To investigate the effect of human cartilage glycoprotein-39 on chondrogenesis of precartilaginous stem cels. METHODS: Precartilaginous stem cels were isolated from the adult articular cartilage. Cels which could express CD105 and CD166 were detected using flow cytometry folowed by isolation and purification. Isolated precartilaginous stem cels werecultured using monolayer method, and then, passage 2 cels were cultured in the medium containing human cartilage glycoprotein-39 and normal chondrogenic medium for 14 days, respectively. Immunohistochemical staining was used to observe expression of type II colagen and gross observation was done for evaluation of cartilage formation. RESULTS AND CONCLUSION:The precartilaginous stem cels isolated from the adult articular cartilage could express CD105 and CD166. After induction, differentiated precartilaginous stem cels gradualy gathered and formed nudes. The induced cels were positive for type II colagen; after induction by human cartilage glycoprotein-39, the nodules became larger and the expression of type II colagen was increased. These findings indicate that precartilaginous stem cels with chondrogenic ability can be isolated from the adult articular cartilage, and can be induced to differentiate into chondrocytes, in which human cartilage glycoprotein-39 plays an important role.
