Effect of Vitamin K2 on Theaortic Artery Calcification in Experimental Rats
10.3969/j.issn.1000-3614.2015.11.016
- VernacularTitle:维生素K2对大鼠主动脉钙化的作用研究
- Author:
Xiaoyu JIANG
;
Donghai ZHANG
;
Anlin LV
;
Huan LI
;
Cuiting QIU
;
Xiaolei MA
;
Xian GUO
;
Shan LI
- Publication Type:Journal Article
- Keywords:
Arterial Calciifcation;
Oxidative stress;
Reactive oxygen species;
Mitochondria
- From:
Chinese Circulation Journal
2015;(11):1101-1105
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effects of Vitamin K2 (VK2) on theaortic artery calciifcation and oxidative stress injury in experimental rats.
Methods: A total of 24 rats were divided into 4 groups:①Control group,②6-week calciifcation group,③12-week calciifcation group and④6-week calciifcation + 6-week VK2 group;n=6 in each group. The arterial calciifcation was induced by warfarin (WFN) treatment. The calcium nodule and deposition in rat’s theaortic artery were detected by Alizarin red staining and o-cresolphthalein complexone method, the reactive oxygen species (ROS) were measured by DHE probe staining and the morphological changes of mitochondria in smooth muscle cells were detected by transmission electron microscopy.
Results: Calciifcation nodule formed in both 6-week and 12-week calciifcation groups, the calciifcation deposition and ROS were higher than Control group,P<0.01. Compared with both calcification groups, the above indexes were decreased in 6-week calciifcation + 6-week VK2 group,P<0.01. Both calciifcation groups showed mitochondria swelling with unclear structure and cytoplasm vacuoles degeneration in vascular smooth muscle cells. The vascular smooth muscle cell volumes were similar between Control group and 6-week calcification + 6-week VK2 group, and no cytoplasm vacuoles degeneration was observed.
Conclusion: Warfarin induced aortic calciifcation is related to oxidative stress injury which may cause the ultra-micro structural damage in smooth muscle cells; VK2 may reduce the oxidative stress injury and improve the condition of vessel calciifcation in experimental rats.
