Calpains are Involved in Entamoeba histolytica-Induced Death of HT-29 Colonic Epithelial Cells.
10.3347/kjp.2011.49.2.177
- Author:
Yun Soo JANG
1
;
Kyoung Ju SONG
;
Ju Young KIM
;
Young Ah LEE
;
Kyeong Ah KIM
;
Sang Kyou LEE
;
Myeong Heon SHIN
Author Information
1. Department of Environmental Medical Biology, Yonsei University College of Medicine, Seoul 120-752, Korea.
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Entamoeba histolytica;
host cell death;
calpain;
HT-29 colon epithelial cells
- MeSH:
Calpain/antagonists & inhibitors/genetics/*metabolism;
*Cell Death;
Cell Line;
Cell Survival/drug effects;
Dipeptides/metabolism;
Entamoeba histolytica/*pathogenicity;
Epithelial Cells/*parasitology;
Gene Knockdown Techniques;
Humans
- From:The Korean Journal of Parasitology
2011;49(2):177-180
- CountryRepublic of Korea
- Language:English
-
Abstract:
Entamoeba histolytica is an enteric tissue-invading protozoan parasite that can cause amebic colitis and liver abscess in humans. E. histolytica has the capability to kill colon epithelial cells in vitro; however, information regarding the role of calpain in colon cell death induced by ameba is limited. In this study, we investigated whether calpains are involved in the E. histolytica-induced cell death of HT-29 colonic epithelial cells. When HT-29 cells were co-incubated with E. histolytica, the propidium iodide stained dead cells markedly increased compared to that in HT-29 cells incubated with medium alone. This pro-death effect induced by ameba was effectively blocked by pretreatment of HT-29 cells with the calpain inhibitor, calpeptin. Moreover, knockdown of m- and micro-calpain by siRNA significantly reduced E. histolytica-induced HT-29 cell death. These results suggest that m- and micro-calpain may be involved in colon epithelial cell death induced by E. histolytica.
