Directional and specific characteristics of astrocyte fiber growth and synapse formation
10.3969/j.issn.2095-4344.2015.42.012
- VernacularTitle:星形胶质细胞纤维生长和突触形成的方向性和特异性
- Author:
Jingli CAO
;
Qi LI
;
Yajie WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(42):6787-6792
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Studies have shown that astrocytes participate not only in the passive metabolic and structural support, but also actively in the central nervous system information processing. OBJECTIVE:By observing the directionality and specificity of astrocyte fiber growth and synapse formation, to explore the possible mechanisms of astrocytes and neurons in the interaction. METHODS: Primary cels from the hippocampi of green fluorescent protein transgenic newborn mice were isolated and cultured, glial fibrilary acidic protein was used as specific marker for astrocytes in immunofluorescence staining, and then the results were observed and scanned by confocal microscope. RESULTS AND CONCLUSION:The elongation of astrocyte fibers was directional and specific, and terminated to the pyramidal cels nearby. The end of astrocyte fibers and pyramidal cels contributed to the synapse formation. However, not al the projections were toward the adjacent cels, and synapse-like structure was not only formed nearby. The results implicated that both astrocytes fiber elongation and synapse formation have the directionality and specificity, providing evidence for further studies on signal transduction between astrocytes and pyramidal cels.
