Changes of Calcium/Calmodulin-dependent Protein Kinase-II in Myocardium Nucleus and Sarcoplasmic Reticulum in Heart Failure Rabbits
10.3969/j.issn.1000-3614.2015.10.018
- VernacularTitle:心力衰竭家兔心肌细胞核及肌浆网钙离子/钙调蛋白依赖性蛋白激酶Ⅱ的变化
- Author:
Fuzheng QU
;
Xiaolu ZHANG
;
Jingwu SUN
;
Xiuhua WANG
;
Aiyan QU
;
Xinlei LU
;
Hongxia ZHOU
;
Lin CHENG
;
Haofei KANG
;
Xiaorui YI
;
Qinghai WANG
;
Jing LIU
;
Mengsong SHI
;
Jing WEI
;
Mingzhe ZHANG
- Publication Type:Journal Article
- Keywords:
Heart failure;
Calcium/calmodulin-dependent protein kinase-II
- From:
Chinese Circulation Journal
2015;(10):1000-1003
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the changes of protein expression and activity of calcium/calmodulin-dependent protein kinase-II (CaMK-II) in myocardium nucleus and sarcoplasmic reticulum in experimental rabbits with heart failure (HF).
Methods: A total of 16 rabbits were divided into 2 groups: Sham group and HF group, the HF model was established by volume overload plus pressure overload.n=8 in each group and all animals were treated for 7 weeks. Left ventricular structure, hemodynamic parameters and protein expression and activity of CaMK-II in myocardium nucleus and sarcoplasmic reticulum were examined and compared between 2 groups.
Results: Compared with Sham group, HF group presented increased left ventricular mass index (LVMI) (1.32 ± 0.06) g/kg vs (3.61 ± 0.09) g/kg, LVEDP (-1.50 ± 0.50) mmHg vs (23.00 ± 2.37) mmHg, allP<0.05; while decreased left ventricular shorten fraction (37.83 ± 3.58) % vs (17.38 ± 3.13) % and LVEF (71.92 ± 4.56) % vs (38.50 ± 6.07) %, allP<0.05. The protein expression and activity of CaMK-II were both higher in HF group than Sham group, allP<0.05.
Conclusion: Increased protein expression and activity of CaMK-II in myocardium nucleus and sarcoplasmic reticulum might be one of the mechanisms for HF occurrence in experimental rabbits.
