Calcium/Calmodulin-dependent Protein Kinase-II δ Involved in Doxorubicin Induced Cardio-toxicity in Experimental Rats
10.3969/j.issn.1000-3614.2015.10.019
- VernacularTitle:钙离子/钙调蛋白依赖性蛋白激酶Ⅱδ参与多柔比星导致的心肌细胞毒性反应
- Author:
Yao QIN
;
Hongyan ZHAO
;
Wenhang ZHANG
;
Dongmei WANG
- Publication Type:Journal Article
- Keywords:
Calcium/Calmodulin-dependent protein kinase-II δ;
Doxorubicin;
Cardio-toxicity
- From:
Chinese Circulation Journal
2015;(10):1004-1007
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the role of calcium/calmodulin-dependent protein kinase-II δ (CaMK-II δ) in doxorubicin (DOX) induced cardio-toxicity in experimental rats.
Methods:①The rat’s cardiomyocytes were treated by DOX and the cell proliferation, protein expression and activity of CaMK-II δ were examined.②CaMK-II δ gene was knocked out by CRISPR method, the changes of DOX induced cell apoptosis and NF-κb activity and miR-146a expression were detected.
Results: DOX could inhibit cardiomyocyte proliferation, the protein expression level of CaMK-II δ was similar and the activity was increased. CRISPR method may effectively knock out CaMK-II δ gene. Compared with normal cells, the cells from CaMK-II δ knocked out rats had decreased sensitivity to DOX induction, suppressed NF-κb activation and miR-146a up-regulation.
Conclusion: CaMK-II δ participated in DOX induced cardio-toxicity in experimental rats and NF-κb and miR-146a were involved in this process.
