H3 K27 me3 demethylase Jmjd3 regulates proliferation and differentiation of embryonal lung epithelia of mice
- VernacularTitle:H3 K27 me3去甲基化酶Jmjd3调节胎鼠肺上皮细胞增殖和分化
- Author:
Huan FENG
;
Chao ZHANG
;
Zhekai ZHANG
;
Ming ZHANG
;
Feng ZHANG
- Publication Type:Journal Article
- Keywords:
Jmjd3;
epithelia of lung;
proliferation;
differentiation
- From:
Basic & Clinical Medicine
2015;(9):1176-1181
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of H3K27me3 demethylase Jmjd3(KDM6B) during the development of lung in embryonal mice .Methods Jmjd3 knockout embryos of E 19.5 mice were examined by HE , PAS and immnohistochemistry assays .Results The developmental defects of the lung of Jmjd 3 heterozygous ( Jmjd3 +/-) embryos were mild is compared to Jmjd3 +/+embryos.However, Jmjd3 -/-mice suffered from the severe hypoplasia of lung tissue .Differentiated defects of ciliated cell , Clara cell , type Ⅰ and Ⅱ alveolar epithelial cells were ob-served in Jmjd3 -/-embryos.The index of cell proliferation was increased in Jmjd3 -/-embryos as compared to wildtype and Jmjd3 +/- embryos.No difference in apoptosis profile was found in these embryos .Conclusions Jmjd3 is essential for proliferation and differentiation of embryonal lung epithelia of mice .
