The effects of silencing Smo gene on proliferation and apoptosis of rat primary chondrocyte
- VernacularTitle:沉默Smo基因对大鼠原代软骨细胞增殖与凋亡的影响
- Author:
Zhijian ZHU
;
Yanni YU
;
Xin TAO
;
Chaonan DENG
- Publication Type:Journal Article
- Keywords:
primary chondrocytes;
lentiviral vector;
siRNA;
apoptosis;
proliferatio
- From:
Basic & Clinical Medicine
2015;(9):1209-1213
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of silencing Smo gene on proliferation and apoptosis of rat prima-ry chondrocyte in vitro.Methods The primary chondrocyte was obtained by mechanical-enzyme digestion and identified by Immunohistochemical cells ( ColⅡ) .The animals were divided into control group , control siRNA group and Smo siRNA 1 ~3 group.The siRNA was transfected into chondrocytes by lentivirus vector .After 72 h, the cell viability was detected by MTT, Smo expression was detected by RT-PCR and Western blot, and the apoptosis of chondrocyte was assessed by flow cytometry .Results All types of siRNA were transfected into primary chondrocyte by vectors, the Smo siRNA 1 ~3 may inhibit the expression of Smo mRNA and protein in chondrocytes, and Smo siRNA2 had the highest silencing rate ( the expressions of Smo mRNA and protein were 0.19 ±0.03 and 0.39 ±0.07 ) .The cell viability in Smo siRNA2 group was lowest ( 77.38% ±7.19%) , while the apoptosis rate of Smo siRNA2 was highest ( 21.43%±2.97%) .Conclusions Silencing Smo gene in primary chondrocytes may inhibit proliferation and promote apoptosis , Smo may have a protecting role from apop-tosis of the chondrocyte.
