The relationship between learning/memory ability and neural cell apoptosis in hippocampal of rats with impaired glucose regulation
- VernacularTitle:糖调节受损大鼠学习记忆能力下降与海马神经细胞凋亡有关
- Author:
Lan ZHAO
;
Yinhua DONG
;
Hongxin WANG
;
Peifen LIANG
- Publication Type:Journal Article
- Keywords:
impaired glucose regulation;
learning and memory;
neuronal apoptosis;
hippocampus
- From:
Basic & Clinical Medicine
2015;(9):1232-1236
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the relationship between impaired glucose regulation of neuronal apoptosis in hip-pocampus and the ability of learning/memory in rats.Methods The model rats were made by high fat and sugar diet;The morris water maze was used to detect the learning and memory function;The TUNEL method was used to detect neuronal apoptosis in hippocampus .Expression of Bcl-2/Bax and Bcl-2 mRNA/Bax mRNA factor in hippo-campus neurons was detected with immunohistochemistry and in situ hybridization .Results Compared with NGT group, in IGR group the learning and memory ability were meaningfully decreased (P<0.05);the number of neuro-nal apoptosis in hippocampus was increased significantly ( P<0.05 ); the expression of Bcl-2 and Bcl-2 mRNA in hippocampus decreased significantly ( P<0.05);the expression of Bax and Bax mRNA in hippocampus increased sig-nificantly ( P<0.05 );The ability of learning and memory was positively correlated with expression of Bcl -2 ( P<0.05) and negatively correlated to the expression of Bax (P<0.05).Conclusions There is a relationship between impaired glucose regulation and the ability of learning and memory in rats , its mechanism may be potentially related to hippocampal neuronal apoptosis .
