Expression of hsa-miR-10 b in three cervical cancer cell lines and its target genes
- VernacularTitle:hsa-miR-10 b在3株宫颈癌细胞系中的表达及靶基因预测
- Author:
Miaomei YU
;
Guanghua LUO
;
Tianhong YU
;
Yang YU
- Publication Type:Journal Article
- Keywords:
bioinformatics;
microRNA;
hsa-miR-10b;
target gene prediction
- From:
Basic & Clinical Medicine
2015;(9):1237-1242
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the expression of hsa-miR-10b in three cervical cancer cell lines , and to find the target genes of hsa-miR-10b.Methods PCR was applied to measure expression levels of hsa-miR-10b in C-33A, HeLa and CaSki .The relative studies on hsa-miR-10b were retrieved from PubMed .The sequence and genome char-acteristics of hsa-miR-10b were analyzed on line by miRbase and NCBI .The target genes of hsa-miR-10b were searched by TargetScan , PicTar and miRanda , and then demonstrated by Gene Ontology and Pathway Enrichment analysis.Results Compared with C-33A, the expression of hsa-miR-10b significantly reduced in HeLa and Caski (P<0.01).Current studies showed that hsa-miR-10b was related with multiple tumorigenesis .hsa-miR-10b was lo-cated in human chromosome 2q31.1 and highly conserved among different species .The target genes were enriched in the biological processes of transcription , gene expression regulation , cell proliferation and etc .(P<0.05).Pathway analysis showed that these target genes were responsible for biochemical erents mediated by to the signaling pathways of cancer, cell cycle, ARF and etc.(P<0.05).Conclusions hsa-miR-10b may have extensive functions , and closely related with the occurrence and development in cervical cancer .Prediction of target genes provides a theoreti-cal basis for the further study .
