Microfluidic chip digital PCR technology and its clinical application
10.3760/cma.j.issn.1009-9158.2015.09.006
- VernacularTitle:微流控芯片数字 PCR 技术及临床应用前景
- Author:
Xiaoyan PAN
;
Zhihua TAO
- Publication Type:Journal Article
- Keywords:
Polymerase chain reaction;
Microfluidic analytical techniques;
Early diagnosis;
Prenatal diagnosis
- From:
Chinese Journal of Laboratory Medicine
2015;(9):592-594
- CountryChina
- Language:Chinese
-
Abstract:
Microfluidics-based digital PCR depends on microfluidic chip to split PCR reaction mixture into many tiny equal-volume units.Quantitative assessment of target DNA template can be obtained by counting the number of fluorescence-positive units after thermocycling.Microfluidics-based digital PCR exhibits many advantages including absolute quantification, high sensitivity and accuracy, and shows great promise in a variety of applications, such as infectious diseases diagnose, early cancer detection and prenatal diagnose. There are already several microfludics-based digital PCR products produced from sereval companies.It is believed that as the technology improves, microfluidics-based digital PCR will find broader applications and become the next-generation tool for genetic tests.
