Roles of maspin in biological behaviors of A549 cells
10.3969/j.issn.1000-4718.2015.09.001
- VernacularTitle:Maspin 在非小细胞肺癌 A549细胞生物学行为中的作用
- Author:
Jun ZHOU
;
Peng ZHOU
;
Hualong QIN
;
Feng GUO
- Publication Type:Journal Article
- Keywords:
Non-small-cell lung cancer;
Maspin;
Cell migration;
Cell invasion
- From:
Chinese Journal of Pathophysiology
2015;(9):1537-1544
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the roles of maspin in the biological behaviors of non-small-cell lung cancer A549 cells.METHODS:Full-length coding region of maspin was amplified by PCR from human normal breast tissue and cloned into MSCV vector .Virus supernatants was produced by Phoenix A packaging system , and target cell line was infec-ted with the virus supernatants .The transfected cells were screened with puromycin .The cell line with maspin over-expres-sion was identified by real-time PCR and Western blot.Cell growth, migration and invasion were investigated by xCelli-gence system .RESULTS:Maspin over-expression vector was successfully constructed .The cell line with maspin over-ex-pression was established .No difference of the growth between A 549-control cells and A549-maspin cells was observed .The migration and invasion were quite different between A 549-control cells and A549-maspin cells.The expression level of inte-grin β1 in A549-maspin cells was decreased compared with A 549-control cells .CONCLUSION:Maspin has no effect on the growth of A549 cells.Maspin suppresses the migration and invasion of A 549 cells, in which integrin β1 is involved.
