Inhibitory role of epigallocatechin-3-gallate in proliferation of human na-sopharyngeal carcinoma cells by targeting P53/miR-34a
10.3969/j.issn.1000-4718.2015.09.004
- VernacularTitle:表没食子儿茶素没食子酸酯通过调控P53／miR－34a 抑制鼻咽癌细胞的增殖
- Author:
Binbin LI
;
Zheng WAN
;
Xia KONG
;
Dan LIAO
;
Ziyou WANG
;
Guoliang HUANG
- Publication Type:Journal Article
- Keywords:
Epigallocatechin-3-gallate;
Nasopharyngeal carcinoma;
P53;
MicroRNA-34a;
Notch1
- From:
Chinese Journal of Pathophysiology
2015;(9):1557-1562
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the effect of epigallocatechin-3-gallate (EGCG) on the proliferation of human naso-pharyngeal carcinoma ( NPC) cells, and to explore its mechanism by targeting miR-34a.METHODS: Nasopharyngeal carcinoma CNE-2Z cells were treated with various concentrations of EGCG .The ability of cell proliferation was detected by CCK-8 assay, 5-ethynyl-2-deoxyuridine (EdU) incorporation assay and colony-forming assay.The cell cycle distributions were analyzed by flow cytometry .The protein levels of P53 and Notch1 were detected by Western blot .The expression of miR-34a and Notch1 mRNA was measured by real-time PCR.RESULTS:EGCG effectively inhibited the proliferation and colony formation of CNE-2Z cells in a dose-dependent manner , which was related to its induction of cell cycle arrest at G 0/G1 phase.The expression of P53 and miR-34a in CNE-2Z cells was significantly increased after treated with EGCG , while the expression of Notch1 at mRNA and protein levels was markedly suppressed .CONCLUSION:EGCG induces cell cycle arrest and suppresses cell proliferation by regulating the P 53/miR-34a/Notch1 pathway in NPC cells.
