tBHQ delayed replicative senescence by activating the proteasome system of BMSCs
10.3969/j.issn.1000-4718.2015.09.021
- VernacularTitle:叔丁基对苯二酚激活骨髓间充质干细胞蛋白酶体活性延缓复制性衰老
- Author:
Huifang SONG
;
Jiachao YANG
;
Xiaojie NIU
;
Li LU
- Publication Type:Journal Article
- Keywords:
Tert-butylhydroquinone;
Bone marrow mesenchymal stem cells;
Replicative senescence
- From:
Chinese Journal of Pathophysiology
2015;(9):1647-1651
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of tert-butylhydroquinone ( tBHQ) on the replicative senescence of bone marrow mesenchymal stem cells (BMSCs).METHODS: Late stage BMSCs were continuously treated with tBHQ at concentration of 30 μmol/L for 4 weeks and the cells were used for the following assays immediately .The proteasomal ac-tivity was determined by chemiluminescence method .The samples were subjected to CCK-8 assay and BrdU incorporation as well as flow cytometry analysis for analyzing the cell vitality and proliferation .Percentage of senescent cells was detected by senescence-associatedβ-galactosidase ( SA-β-Gal) staining.The expression of P53 was measured by Western blot .RE-SULTS:After the continuous treatment of tBHQ (30 μmol/L) for 4 weeks, the proteasomal activity of late stage BMSCs increased by 21.96%±1.98%(P<0.05).The cell vitality and survival were significantly increased with the increases in tBHQ doses till 40 μmol/L, and no cytotoxicity reaction with the increased dose of tBHQ till 120 μmol/L was observed . BrdU-positive cells, which represented the cell proliferation , were significantly increased (P<0.05).The proliferation in-dex was also significantly increased by flow cytometry analysis (P<0.05).The SA-β-Gal positive cells and the expression of P53 were decreased (P<0.05).CONCLUSION:tBHQ delays the proteasome dysfunction associated senescence pro-gress of BMSCs by increasing the proteasomal activity .
