Effect of Egr-1 gene transfection on renal inflammation in diabetic mice
10.3969/j.issn.1000-4718.2015.09.028
- VernacularTitle:Egr－1基因转染对糖尿病小鼠肾脏炎症反应的影响
- Author:
Jie LIU
;
Haisong ZHANG
;
Yongjun MA
;
Li LIU
;
Minghui HOU
;
Hongjie WANG
;
Hui YANG
- Publication Type:Journal Article
- Keywords:
Early growth response protein 1;
Gene transfection;
Diabetic nephropathies;
Tumor necrosis fac-tor-α;
Intercellular adhesion molecule-1
- From:
Chinese Journal of Pathophysiology
2015;(9):1688-1692
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effects of Egr-1 gene transfection on the expression of tumor necrosis factor-α( TNF-α) and intercellular adhesion molecule-1 ( ICAM-1) , and to investigate the role of Egr-1 in the pathogenesis of dia-betic nephropathy .METHODS:The diabetic mouse model was established .Ten mice were randomly selected as the dia-betic group .The remaining 40 mice were injected with empty plasmid , Egr-1 expression plasmid or Egr-1 siRNA plasmid via the tail vein once a week.The normal control group was also set up .The animals were sacrificed at the end of the 4th week.The renal tissues were harvested .The expressions of Egr-1, TNF-αand ICAM-1 were detected by immunohisto-chemistry and Western blot .The pathological changes were observed under electron microscope .RESULTS: In diabetic mouse kidney, the expression of Egr-1, TNF-αand ICAM-1 was increased, and irregular thickening of glomerular basement membrane , mesangial expansion and fusion of foot were observed .The change trend was more significant in Egr-1 gene transfection group , and these changes in siRNA plasmid transfection group were obviously reduced compared with diabetes group.CONCLUSION:Egr-1 up-regulates the expression of TNF-αand ICAM-1, and induces mesangial cell proliferation and mesangial extracellular matrix accumulation , which is probably one of the mechanisms of accelerating glomerulosclerosis .
