Inhibitory effect of catalpol on inflammation and expression of RAGE in EA.hy926 cells induced by advanced glycation end products
10.3969/j.issn.1000-4718.2015.09.029
- VernacularTitle:梓醇抑制 AGEs 诱导的 EA．hy926细胞炎症反应及 RAGE 表达
- Author:
Jiangyue LIU
- Publication Type:Journal Article
- Keywords:
Catalpol;
Oxidative stress;
Inflammation;
Receptor for advanced glycation end products
- From:
Chinese Journal of Pathophysiology
2015;(9):1693-1698
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the inhibitory effect of catalpol on inflammation in EA .hy926 cells induced by advanced glycation end products ( AGEs) and to explore its antioxidant mechanisms .METHODS:Human endothelial cell line EA.hy926 was cultured and randomly divided into control group , catalpol (0.5 mmol/L) group, AGEs group, high-dose (0.5 mmol/L) catalpol +AGEs group, middle-dose (0.25 mmol/L) catalpol +AGEs group and low-dose (0.05 mmol/L) catalpol+AGEs group.Intracellular reative oxygen species ( ROS) production was detected by laser scanning confocal microscopy.The levels of monocyte chemotactic protein-1 (MCP-1), tumor necrosis factor-α(TNF-α) and vas-cular cell adhesion molecule-1 (VCAM-1) in culture supernatant were detected by commercial ELISA kits .The expression of MCP-1, TNF-α, VCAM-1 and receptor for advanced glycation end products (RAGE) in the EA.hy926 cells were detec-ted by Western blot.RESULTS:In high-dose catalpol+AGEs and middle-dose catalpol+AGEs groups, the generation of ROS was decreased significantly .The levels of MCP-1, TNF-αand VCAM-1, and protein expression of MCP-1, TNF-αand VCAM-1 were significantly lower .The expression of RAGE protein in EA .hy926 cells were significantly inhibited ( P<0.05).CONCLUSION:Catalpol effectively inhibits the AGEs-induced oxidative stress and inflammation in EA .hy926 cells, which may be associated with a decrease in the expression of RAGE .
