Effects of lentivirus-mediated RWDD3 silencing on proliferation and inva-sion of human glioma U251 cells
10.3969/j.issn.1000-4718.2015.09.003
- VernacularTitle:慢病毒介导的 RWDD3沉默对人胶质瘤 U251细胞增殖和侵袭的影响
- Author:
Yanghua FAN
;
Xingen ZHU
;
Miaojing WU
;
Yi CHAI
;
Minhua YE
;
Bing XIAO
;
Lei WU
- Publication Type:Journal Article
- Keywords:
Glioma;
RWDD3;
Lentivirus;
Proliferation;
Invasion
- From:
Chinese Journal of Pathophysiology
2015;(9):1550-1556
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of RWDD3 gene silencing on the biological characteristics of human glioma U251 cells.METHODS: A lentiviral vector expressing RWDD3 shRNA was constructed and transfeeted into the U251 cells.The expression of RWDD3 at mRNA and protein levels was detected by real-time PCR and Western blot , re-spectively .The cell activity was determined by MTT assay .The colony formation ability was detected by the colony forma-tion assay .The cell proliferation ability was detected by BrdU incorporation assay .The cell invasion and migration were evaluated by Transwell assay .Flow cytometry was used to monitor the changes of cell cycle distribution and apoptosis .RE-SULTS:Recombinant lentivirus was successfully transfected into U 251 cells.Compared with the cells transfected with the scrambled shRNA and control cells, the cell activity, colony formation ability, and the invasive and migratory activities were inhibited, the cell cycle was arrested in G 0/G1 phase, and the apoptosis was increased in the U 251 cells transfected with RWDD3 shRNA ( P <0.05 ) .CONCLUSION: RWDD3 plays a vital role in proliferation and invasion of glioma cells.It may serve as a potential target of gene therapy for glioma .
