Mechanism of Herceptin enhancing apoptosis and improving sensitivity to chemotherapy in Ishikawa cells
10.3969/j.issn.1000-4718.2015.09.006
- VernacularTitle:赫赛汀诱导子宫内膜癌 Ishikawa 细胞凋亡并增强其对化疗的敏感性
- Author:
Yuan SHEN
;
Lin LU
;
Xiaoyu WANG
- Publication Type:Journal Article
- Keywords:
Herceptin;
Endometrial cancer;
Chemotherapeutic sensitivity;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2015;(9):1568-1571
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the role of Herceptin in the apoptosis and drug sensitivity of endometrial canc -er Ishikawa cells .METHODS: The IC50 values of Herceptin , adriamycin ( ADR ) , cisplatin ( DDP ) and paclitaxel ( PTX) for Ishikawa cells were detected by MTT method .Ishikawa cells were treated with single drug and combined chemo-therapy for 24 h, the cell cycle and the apoptosis ratio were determined by flow cytometry .RESULTS:The IC50 values of Herceptin, ADR, DDP and PTX were 57.12 mg/L, 0.572μmol/L, 67.4μmol/L and 719.5 nmol/L, respectively.Her-ceptin significantly enhanced the cytotoxicity of the chemotherapeutic drugs , and increased apoptosis ratio statistically . CONCLUSION:Herceptin enhances the apoptosis-inducing ability of the chemotherapeutic drugs and improves the che-motherapeutic sensitivity in Ishikawa cells .
