Resveratrol regulates iNOS to inhibit atherosclerosis in C57BL/6J mice
10.3969/j.issn.1000-4718.2015.09.012
- VernacularTitle:白藜芦醇通过调节 iNOS 抑制 C57BL／6J小鼠动脉粥样硬化的机制探讨
- Author:
Li QIN
;
Li GUAN
;
Ruishan LIN
;
Qianqian XUE
;
Jinwen XU
;
Fuman YAN
;
Xiaoying LI
;
Haimei LIU
- Publication Type:Journal Article
- Keywords:
Resveratrol;
Atherosclerosis;
Nitric oxide;
Inducible nitric oxide synthase
- From:
Chinese Journal of Pathophysiology
2015;(9):1601-1605
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of resveratrol on the lipids ( CHOL, TG, LDL-C and HDL-C) , ni-tric oxide ( NO) , peroxynitrite anion ( ONOO-) and the expression of inducible nitric oxide synthase ( iNOS) in the artery of the mice with ovariotomy ( OVX) .METHODS:The lipid levels and NO level in the serum were measured .The chan-ges of atherosclerosis were evaluated with Oil Red O staining .The expression of iNOS was measured by DAB staining and Western blot .The ONOO-production was measured by DAB staining .RESULTS:Compared with sham group , the levels of the lipids and NO production in OVX +high fat (HF) group were increased (P<0.05).Compared with OVX+HF group, the levels of the lipids and NO production in resveratrol group were decreased (P<0.05).Fourteen weeks later, the atherosclerosis model was successfully established .Compared with OVX +HF group, the iNOS expression and the ONOO-production in resveratrol group were decreased ( P<0.05 ) , while those in sham group were increased ( P <0.05).CONCLUSION:Resveratrol prevents and treats atherosclerosis by inhibiting the iNOS expression in C 57BL/6J mice.
