Overexpression of neuroglobin attenuates Aβ-induced apoptosis in brains of double transgenic AD mice
10.3969/j.issn.1000-4718.2015.09.016
- VernacularTitle:过表达脑红蛋白抑制双转基因 AD 小鼠脑中 Aβ诱导的凋亡
- Author:
Jun YANG
;
Songyang DAI
;
Yu LI
;
Xiong ZHANG
- Publication Type:Journal Article
- Keywords:
Neuroglobin;
Caspases;
pI3K/Akt pathway;
Alzheimer disease
- From:
Chinese Journal of Pathophysiology
2015;(9):1621-1626
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effects of neuroglobin ( NGB) overexpression on the apoptosis induced by Aβin the brains of double transgenic AD ( APPswe/PS1dE9) mice and to explore its potential mechanisms .METHODS:Twenty-four 13-month-old double transgenic AD mice were randomly divided into 3 groups:intracerebroventricular injection with normal saline (NS) group, intracerebroventricular injection with pcDNA3.1 and NS group, and intracerebroventricular injection with pcDNA3.1 and pNGB group.Immunohistochemistry was used to detect the expression of Aβ1-42 in the brains. TUNEL staining was used for analyzing the apoptosis , and the protein levels of cleaved caspase-3, caspase-9, PI3K, Akt and p-Akt were determined by Western blot .RESULTS: After intracerebroventricular injection with pNGB , the areas of Aβ1-42 in the hippocampus and cortex were decreased compared with NS group and pcDNA 3.1+NS group (P<0.01).The TUNEL-positive staining cells in the pNGB group were less than those in NS group and pcDNA 3.1 group ( P<0.01 ) . NGB overexpression attenuated the protein levels of cleaved caspase-3 and caspase-9 (P<0.01), but induced the produc-tion of PI3K and p-Akt (P<0.01).CONCLUSION:Overexpression of pNGB significantly inhibits the generation of Aβand attenuates the apoptosis induced by Aβ, indicating that NGB overexpression activates PI 3K/Akt pathway and inhibits the production of cleaved caspase-3 and caspase-9, which were tightly related with apoptosis .
