Effect of Ginsenoside Rh2 on apoptosis of human osteosarcoma cell line MG-63
10.3969/j.issn.1000-4718.2015.09.033
- VernacularTitle:人参皂苷 Rh2对人骨肉瘤细胞 MG－63凋亡的影响
- Author:
Limin WANG
- Publication Type:Journal Article
- Keywords:
Ginsenoside Rh2;
Human osteosarcoma cell line MG-63;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2015;(9):1715-1719
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of Ginsenoside Rh2 (Rh2) on the apoptosis of human osteosarcoma cell line MG-63.METHODS:The cell viability was determined by MTT assay .MG-63 cell apoptotic rate was examined by flow cytometry with Annexin V-PI double staining.The expression of Bcl-2, Bax, cytochrome C ( Cyt C) and cleaved caspase-3 were measured by Western blot .RESULTS: Rh2 enhanced the apoptosis of MG-63 cells in a dose-dependent manner.Furthermore, after treatment with Rh2, the release of mitochondrial Cyt C and Bax expression were increased , while Bcl-2 and the ratio of Bcl-2/Bax were decreased as compared with control group ( P<0.05 ) .The protein level of cleaved caspase-3 was also increased (P<0.05).CONCLUSION:Ginsenoside Rh2 accelerates the apoptosis of MG-63 cells through mitochondria-dependent pathway , suggesting that Rh2 is a novel approach for the treatment of osteosarcoma .
