Tanshinone IIA prevents high glucose-induced human umbilical vein en-dothelial cell apoptosis
10.3969/j.issn.1000-4718.2015.09.034
- VernacularTitle:丹参酮 IIA 对高糖诱导人脐静脉内皮细胞凋亡的作用
- Author:
Jianxin YU
;
Qi WU
;
Huan YANG
- Publication Type:Journal Article
- Keywords:
Tanshinone IIA;
High glucose;
Endothelial cells;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2015;(9):1720-1723
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of tanshinone IIA on the apoptosis of human umbilical vein endothe -lial cells (HUVECs) after high glucose treatment.METHODS:The cell viability was determined by MTT assay.The cell apoptotic rate was examined by flow cytometry with Annexin V-FITC/PI double staining .The expression of Bcl-2 and Bax, and the release of mitochondrial cytochrome C ( Cyt C) were analyzed by Western blotting .RESULTS: Tanshinone IIA significantly inhibited high glucose-induced decrease in cell viability and increased the cell apoptosis .Additionally, after tanshinone IIA treatment , Bax expression and the release of mitochondrial Cyt C were significantly inhibited , while Bcl-2 expression was increased .CONCLUSION: Tanshinone IIA prevents high glucose-induced endothelial cell apoptosis via mitochondria-dependent pathway .
