Associations between HLA-G gene polymorphisms and the susceptibility to high risk HPV type 18 in-fection
10.3760/cma.j.issn.0254-5101.2015.08.008
- VernacularTitle:HLA-G基因多态性与高危型HPV18易感的相关研究
- Author:
Huihui XU
;
Lili CHEN
;
Yang ZHANG
;
Meiying ZHOU
;
Weihua YAN
- Publication Type:Journal Article
- Keywords:
HLA-G gene polymorphism;
Linkage disequilibrium;
Human papillomavirus;
Cervi-cal intraepithelial neoplasia
- From:
Chinese Journal of Microbiology and Immunology
2015;(8):595-599
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the associations between single nucleotide polymorphisms in the gene encoding human leukocyte antigen-G ( HLA-G) and the genetic susceptibility to high risk human papillomavirus ( HPV) type 18 infection in the subjects from Taizhou, Zhejiang province. Methods The genetic polymorphisms of HLA-G gene (14 bp In/Del and +3142C/G) in cervical samples collected from HPV 18-positive and healthy women were analyzed by PCR and gene sequencing technology. Statistical anal-ysis was performed by using SPSS version 16. 0. Chi-squared test was used to analyze the differences with HLA-G gene allele and genotype frequencies between healthy subjects and patients. Results Compared with healthy subjects, women with oncogenic HPV18 infection showed lower frequencies of -14 bp allele,-14 bp/-14 bp genotype and -14 bp/+3142C haplotype (P<0. 05). Moreover, lower frequencies of+3142C/C genotype were detected in HPV18-infected women with pathologically normal cervix (6. 3% vs 21. 1%, OR=0. 25, P<0. 05) and higher percentages of +3142G/G genotype were detected in women with CIN2/3 stage HPV18 infection as compared with those of the control group (68. 8% vs 35. 1%, OR=4. 06, P<0. 05). Conclusion The HLA-G gene 3′ UTR polymorphisms were closely associated with HPV18 in-fection and cervical intraepithelial neoplasia.
