Study of immune function of dendritic cells in peripheral blood of tuberculosis patients after initial treatment
10.11958/j.issn.0253-9896.2015.10.018
- VernacularTitle:初治肺结核患者外周血树突状细胞免疫功能的体外研究
- Author:
Yi XU
;
Li YANG
;
Lei ZHAO
;
Jinfeng CAO
;
Wei HAN
;
Zhi ZHANG
;
Huixia GAO
;
Yuzhen LIU
;
Erhei DAI
- Publication Type:Journal Article
- Keywords:
dendritic cells;
tuberculosis,pulmonary;
cytokines;
flow cytometry
- From:
Tianjin Medical Journal
2015;(10):1152-1155
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate expressions of surface maturation markers, secreting cytokines and the stimulat?ing effect on T lymphocytes in the dendritic cells (DC) from peripheral blood of tuberculosis (TB) patients after loading dose treatment. Methods TB patients who received initial treatment (n=68) were collected at the fifth hospital of Shijiazhuang from 2013 June to 2014 January. Base on clinical diagnosis and treatment guidelines of tuberculosis, they were divided into sputum smear-negative group (35 cases) and sputum smear-positive group(33 cases). Forty cases of healthy adult were se?lected as control group. Mononuclear cells were isolated from peripheral blood and were cultured in medium to differentiate into DCs. Expression levels of CD83 and CD86 on DCs were examined by flow cytometry. The proliferation of allogeneic mixed lymphocyte stimulated by DCs was dectected using MTT assay. Contents of IL-12, IL-10 and INF-γin the cultural supernatant of DCs and blood serum from TB patients were detected by ELISA. Results Compared with controls ,the ex?pressions of CD83 and CD86 on DCs in TB patients after loading dose treatment decreased obviously(P<0.05), and the ability to stimulate the proliferation of lymphocytes reduced evidently(P<0.05). What’s more, IL-12 level increased mark?edly(P<0.05)while IL-10 and INF-γlevels presented no significant difference among the three groups (P>0.05). Conclu?sion The expressions of maturation markers of DC cells of the peripheral blood in TB patients after initial treatment de?creased. The ability of stimulating mixed lymphocyte proliferation is also significantly reduced while secretion of IL-12 was enhanced.
