Effects of Hedan tablet on cytokins and oxidative stress factors in APOE-/-mouse
10.11958/j.issn.0253-9896.2015.10.016
- VernacularTitle:荷丹片对APOE-/-小鼠炎症因子及氧化应激因子的影响
- Author:
Baiqing YU
;
Yujuan ZHOU
;
Fulin LIU
;
Xiaohua CAO
;
Mingxuan XU
- Publication Type:Journal Article
- Keywords:
atherosclerosis;
apolipoproteins E;
models,animal;
Hedan tablet;
APOE-/-mouse;
inflam-mation factor;
oxidation stress factor
- From:
Tianjin Medical Journal
2015;(10):1144-1146
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Hedan tablet on cytokines and oxidation factors in APOE-/-mouse, and to explore its effect on atherosclerosis and to explore its behind mechanism. Methods APOE-/-mice (n=50) were randomly divided into control group, model group, low dose Hedan tablet treatment group, high dose Hedan tablet treatment group and simvastatin treatment group. Mice in control group were given normal feed while mice in other groups were fed with high cho?lesterol diet. Hedan or Simvastatin was administrated intra-gastrically while normal saline was given to model group in the same route. After 12 weeks, mice were sacrificed to observe the mRNA level of tumor necrosis factor-α(TNF-αmRNA) in aorta by RT-PCR. Mean while, serum levels of interleukin-1 (IL-1), interleukin-10 (IL-10), malonaldehyde (MDA) and su?peroxide dismutase (SOD) were determined in different groups. Results Compared with control group, TNF-αmRNA tran?scription level as well as serum levels of IL-1 and MDA significantly increase while serum levels of IL-10 and SOD de?creased remarkably in model group, (P<0.01). Compared with model group, mRNA levels of TNF-αas well as serum levels of IL-1 and MDA were significantly decreased while serum levels of IL-10, SOD were greatly increased in low dose and high dose Hedan tablet treatment groups as well as in simvastatin treatment group (P<0.01). Conclusion Hedan tablet inhibit the formation of atherosclerosis through its anti-oxidation role and anti-inflammation role.
