Screening, identification and function of pPCP1 plasmid-derived sRNA in Yersinia pestis:a preliminary study
10.7644/j.issn.1674-9960.2015.09.006
- VernacularTitle:鼠疫耶尔森菌外源获得性pPCP1质粒编码小RNA的筛选、鉴定与功能的初步研究
- Author:
Hongduo WANG
;
Zizhong LIU
;
Ziying WANG
;
Ruifu YANG
;
Yanping HAN
- Publication Type:Journal Article
- Keywords:
Yersinia pestis;
pPCP1;
sRNA;
biofilm;
virulence
- From:
Military Medical Sciences
2015;(9):672-676
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify small non-coding RNAs encoded by plasmid pPCP1 and investigate their roles in biofilm formation, stress tolerance and/or virulence in Yersinia pestis.Methods Seven plasmid pPCP1-encoded sRNAs were identified by RNA-seq results in Y.pestis in our previous studies.Northern blot was used to validate the presence of the seven sRNAs.The sRNA-deletion mutants were constructed via λ-Red homologous recombination system.The biofilm formation, high salt tolerance and virulence of the phenotypes were compared between Y.pestis WT strain and sRNA mutants.Results and Conclusion The expression of seven pPCP1-encoded sRNAs was validated and the transcript length detected by Northern blotting corresponded to the length observed by RNA-seq.On this basis, five sRNA-deletion mutants were obtained.The capacity of biofilm formation was weakened upon deletion of sR3446.The tolerance of sR3446, sR3457, sR4338 and sR4340 mutants was found weakened in vitro compared to that of wild-type strain,but the tolerance of sR6143 was found increased.Slight virulence attenuation was found in two sRNA mutants ( sR4338 and sR4340 ) .The results suggest that pPCP1-deriving sRNA might be implicated in stress response, biofilm and virulence in Y.pestis.
