Roles of BTG3 Expression in Gastric Cancer and Mechanism for Its Tumor Suppression Function
- VernacularTitle:胃癌中BTG3表达的临床病理意义及抑瘤作用的机制
- Author:
Huachuan ZHENG
;
Daofu SHEN
;
Xuefeng YANG
;
Wenfeng GOU
;
Shuang ZHAO
;
Wenlu ZHANG
- Publication Type:Journal Article
- Keywords:
gastric cancer;
BTG3;
carcinogenesis;
pathobiological behaviors;
gene therapy
- From:
Journal of China Medical University
2015;(10):877-882
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clarify the clinicopathological significance and the reversing effects of BTG3 expression on the aggressive phenotype in gastric cancer. Methods BTG3 expression was detected in gastric cancer tissues by on tissue microarray and immunostaining. BTG3?expressing plasmid was transfected into MKN28 and MGC803 cells,the proliferation,cell cycle,differentiation and autophagy were analyzed by CCK?8,PI staining,alkaline phosphatase activity and GFP?LC?3B transfection,respectively. Results BTG3 overexpression inhibited cell proliferation,in?duced S/G2 arrest,differentiation and autophagy in both cells(P<0.05). BTG3 expression was decreased in gastric cancer in comparison with the adjacent mucosa(P<0.05),and positively correlated with venous invasion and dedifferentiation of the cancers(P<0.05). Conclusion BTG3 ex?pression contributes to gastric carcinogenesis and subsequent progression. BTG3 overexpression can reverse the aggressive phenotypes,which could be employed as a potential target for gene therapy of gastric cancer.
