Application of Magnetic Beads Method for Methylated ctDNA Detection in Urine
- VernacularTitle:应用磁珠法检测并提取尿液游离甲基化DNA
- Author:
Ning SUN
;
Jialin ZHANG
;
Xiangyu ZHOU
;
Chengshuo ZHANG
;
Rui YU
;
Bochao ZHAO
- Publication Type:Journal Article
- Keywords:
magnetic beads;
methylation;
urine methylation ctDNA
- From:
Journal of China Medical University
2015;(10):897-900
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simple method to extract the methylated ctDNA in urine using magnetic beads as solid phase adsorption carri?er with a specific design reagent system and extraction process,and evaluate its application feasibility for methylated gene detection in urine sample . Methods Fourty cases of methylated ctDNA were extracted in urine using magnetic beads. After methylated modification,the concentration and pu?rity of DNA was determined by ultraviolet spectrophotometer. Results The extraction of 50 mL urine can gain 61?200 ng/mL methylated ctDNA, and the OD260/280 was 1.8 ± 0.05. Conclusion There are methylated ctDNA exist in the urine which can be extracted by magnetic beads. The estab?lished extraction method is simple,effective,and with high purity.
