Comparison of the effects of astragaloside and NMDA receptor antagonist on the impairment of learning -memory after the fetal intrauterine distress in neonatal rats
10.3760/cma.j.issn.1008-6706.2015.19.001
- VernacularTitle:黄芪总苷和 N-甲基-D-天冬氨酸受体拮抗剂缓解宫内窘迫后新生鼠学习记忆障碍
- Author:
Shan ZHU
;
Chao LIU
- Publication Type:Journal Article
- Keywords:
Fetal distress;
Rats;
Astragaloside;
Receptors,N-Methyl-D-Aspartate;
Protein Tau
- From:
Chinese Journal of Primary Medicine and Pharmacy
2015;(19):2881-2885
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the reversion of astragaloside and NMDA receptor antagonist against the hyperphosphorylation of protein Tau induced by fetal intrauterine distress in neonatal rats.Methods The analysis of variance of factorial design was setted up two intervention factors which were fetal intrauterine distress (two levels:no disposition;a course of fetal intrauterine distress)and the drugs (three levels:iv Saline;iv astragaloside;iv MK -801).When the neonatal rats grew to 12weeks,the hippocampus was removed from the neonatal rats.Detected the content of glutamate in the hippocampus of rats by high performance liquid chromatography.The expression of protein Tau which includes p -AT8Ser202 and GSK -3β1H8 in the hippocampus of rats were detected by the methods of immunohistochemistry staining.Results Fetal intrauterine distress could significantly up -regulate the content of glutamate,which was not affected by MK -801,in the hippocampus of neonatal rats which was reduces by the astragaloside (P <0.05).And both influences presented subtracting effects (P <0.05).Fetal intrauterine distress and the drugs do not affect the total protein Tau in the hippocampus of rats (P <0.05).Fetal intrauterine distress could up -regulate the hyperphosphorylation of protein Tau in the hippocampus of neonatal rats which could be reduced by astragaloside and MK -801 (P <0.05).And the influences between fetal intrauterine distress and the drugs presented subtracting effects (P <0.05 ).Conclusion Our results indicate that fetal intrauterine distress reduce the hyperphosphorylation of protein Tau in neonatal rats though up -regulating the content of glutamate.GSK-3βis the key protein in this signaling pathway.
