The study of the apoptosis induced by EPA on human colon cancer SW480 cells
10.3760/cma.j.issn.1008-6706.2015.19.010
- VernacularTitle:二十碳五烯酸诱导人结肠癌 SW480细胞凋亡机制研究
- Author:
Xiangqun WANG
;
Lijuan CHEN
;
Qing ZHANG
;
Dan WU
;
Junhua LUO
;
Jian ZHU
- Publication Type:Journal Article
- Keywords:
Colon tumor;
Twenty carbon five acid;
SW480 cells;
Cell apoptosis
- From:
Chinese Journal of Primary Medicine and Pharmacy
2015;(19):2913-2915
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of eicosapentaenoic acid(EPA)on the apoptosis of human colon cancer SW480 cells and the mechanisms.Methods Mitochondrial membrane potential was detected,the quan-tity of cytochrome C was analyzed by Elisa kit,and the expression of cleaved caspase -9 and caspase -3 was detected by Western Blot.Results After treatment with EPA (0μg/mL,42.1μg/mL,84.2μg/mL,168.4μg/mL),the mitochondrial membrane potential(Δψm)of SW480 cells were declined (P <0.05 ),the values were (99.71 ± 0.04)%,(95.04 ±0.10)%,(88.65 ±0.41)% and (73.60 ±1.20)%(t =5.161,6.302,4.601,5.198,all P <0.05).The quantity of cytochrome C in cytosol was increased significantly compared with no treatment group,and the values were (12.8 ±1.2)ng/mL,(115.5 ±3.5)ng/mL,(290.5 ±5.2)ng/mL and (262.0 ±12.5 )ng/mL in different EPA treatment groups(t =6.345,6.013,5.846,4.613,all P <0.01).The expression of cleaved caspase -9 and caspase -3 were significantly increased.Conclusion EPA inhibits SW480 cells growth and induces apoptosis in a dose dependent manner.This action may be mediated by mitochondria -mediated intrinsic apoptosis pathway,cyto-chrome C release,and caspase -9 and caspase -3 activation.
