Influence of Azithromycin on peripheral blood helper T lymphocyte 9 cells and helper T lymphocyte 17 cells in ;children with bronchial asthma
10.3760/cma.j.issn.2095-428X.2015.18.012
- VernacularTitle:阿奇霉素对支气管哮喘患儿外周血辅助性T淋巴细胞9和辅助性T淋巴细胞17功能的影响
- Author:
Wei LIU
;
Juan ZHOU
;
Ying SUN
;
Qiuye ZHANG
- Publication Type:Journal Article
- Keywords:
Helper T lymphocyte 9;
Helper T lymphocyte 17;
Interleukin-9;
Interleukin-17;
Azithromycin;
Bronchial asthma
- From:
Chinese Journal of Applied Clinical Pediatrics
2015;(18):1405-1408
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of Azithromycin on helper T lymphocyte 9 cells ( Th9 ) and Th17 in peripheral blood of children with bronchial asthma,and to investigate the immunomodulating effect of Azithro-mycin. Methods Twenty-six asthmatic children were selected as the experimental group,and 17 healthy children as a control group. Peripheral blood mononuclear cells(PBMC)were isolated from venous blood by density gradient cen-tri-fugation under aseptic conditions. PBMC were split by phytohemagglutinin( PHA) in vitro. Different concentrations of Azithromycin (0,0. 1,1. 0,10. 0 mg/L)were added into the cultures in the experimental group. The control group was not interfered with azithromycin. The supematant was collected after 72 h. The levels of interleukin ( IL)-4, IL-9,IL-17 and IL-23 in the supematant were determined by enzyme-linked immunosorbent assay(ELISA). SPSS 17. 0 software was used to analyze data. Results (1) The levels of IL-4,IL-9,IL-17 and IL-23 produced from PBMC of the experimental group were significantly higher than those in the control group(t=9. 210,3. 040,8. 965,2. 796,P<0. 05). (2) The levels of IL-9 and IL-4 in Azithromycin 10. 0 mg/L were significantly lower than those in the Azithromycin 0 mg/L(P<0. 05). But there were no differences among other groups(P>0. 05). The levels of IL-17 and IL-23 of Azithromycin 10 mg/L were lower than those in Azithromycin 0 mg/L and 0. 1 mg/L(P<0. 05),but there were no significant differences among other groups(P>0. 05). (3)IL-9 level was negatively correlated with IL-4 level in the experimental group(r=-0. 255,P>0. 05). The levels of IL-23 and IL-17 secreted by Th17 in asthmatic chil-dren had correlation. The secretion of IL-17 levels rose with the secretion of IL-23 rise(r=0. 64,P<0. 05). Conclu-sions The function of Th9 and Th17 in asthmatic children was enhanced;Azithromycin could restrain the function of Th9 and Th17 at higher tissue concentrations (10. 0 mg/L),the former was unrelated to changes in the secretion of IL-9 levels,and the latter with the secretion of IL-23 levels decreased close. Azithromycin can play a role of immune modulators in higher concentrations,inflammatory cytokines are inhibited in the asthmatic children,and Azithromycin relieves airway inflammation.
