Effect of Acupuncture on the Expression of NOS in Gastrointestinal Tissues after Abdominal Operation in Rats
10.13460/j.issn.1005-0957.2015.09.0888
- VernacularTitle:针刺对大鼠腹部术后胃肠组织中NOS表达的影响
- Author:
Fang ZHENG
;
Ping LI
;
Fanzheng MENG
;
Jinsheng ZHAO
- Publication Type:Journal Article
- Keywords:
Acupuncture therapy;
Intestinal obstruction,Incomplete;
NOS;
Rats
- From:
Shanghai Journal of Acupuncture and Moxibustion
2015;(9):888-891
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of acupuncture on the expression of nitricoxide synthase (NOS) in gastrointestinal tissues after operation for incomplete intestinal obstruction in rats, and to explore the mechanism of acupuncture in promoting the recovery of gastrointestinal motility. Methods Forty Sprague-Dawley (SD) rats were randomized into treatment group 1, treatment group 2, model group 1, model group 2, and a normal control group, 8 rats in each group. The incomplete intestinal obstruction models were developed by using single ligation method in treatment groups and model groups. Afterwards, the treatment groups were intervened by acupuncture at Zusanli (ST 36) and Yanglingquan (GB 34), and rats in treatment group 1 and 2 were respectively dissected after 1-day and 2-day treatment. The rats in model group 1 and 2 were respectively dissected 1 d and 2 d after modeling. The morphological observation was performed on rat’s intestinal tissues, and the average diameter of the upper part of the obstructed intestine and the expression of NOS in gastrointestinal tissues were compared. Results The average ileocecal diameter after modeling was significantly different from that in the normal control group (P<0.05). There was a significant difference in comparing the ileocecal diameter between model group 1 and 2 (P<0.05). The ileocecal diameter in treatment group 1 was significantly different from that in treatment group 2 and model groups (P<0.05). The ileocecal diameter in treatment group 2 was insignificantly different from that in the normal control group (P>0.05). The NOS content in model groups was significantly different from that in the normal group (P<0.05). The NOS content in treatment group 1 was markedly different from that in model groups and treatment group 2 (P<0.05) after intervention. There were no significant inter-group differences in comparing NOS content (P>0.05). Conclusions Acupuncture can promote the recovery of gastrointestinal motility by regulating intestinal NOS content in rats after operation for incomplete intestinal obstruction.
