Establishment and pathophysiological changes of a rat model of increased blood flow-induced pulmonary arterial hypertension by anastomosis of the left common carotid artery to left external jugular vein
10.3969/j.issn.1671.7856.2015.009.007
- VernacularTitle:大鼠高血流性肺动脉高压模型建立及病理生理改变
- Author:
Keyan ZHAO
;
Hongyan LI
;
Huishan WANG
;
Changci TONG
;
Yubiao ZHANG
;
Lin SHI
;
Mingxiao HOU
- Publication Type:Journal Article
- Keywords:
Hypertension,pulmonary artery;
Animal model;
Rat;
Echocardiography
- From:
Chinese Journal of Comparative Medicine
2015;(9):33-38
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveToestablisharatmodelofincreasedbloodflow-inducedpulmonaryarterialhypertension generatedbyanastomosisoftheleftcommoncarotidarterytoleftexternaljugularvein.Methods 45maleSDratswere divided into three groups:the shunt group , the ligation group and the sham group .At twelve weeks after the procedure , the general status of the rats was observed . Heart conditions , cardiac output and shunt patency were measured by echocardiography .Right ventricular systolic pressure ( RVSP ) and Qp/Qs were checked by catheterization . Right ventricular hypertrophy index ( RVHI) was calculated and lung tissues were examined by pathology using hematoxylin -eosin and elastin Van Gieson staining .All data were analyzed statistically by one-way ANOVA test using SPSS 16.0.Results There was no significant difference in body weight gains between the groups .The patency rate of shunt was 84.6%.The heart was enlarged in the group shunt .Cardiac output increased significantly in the shunt group than that in the other two groups [(309.8 ±33.1) mL/min?kg vs.(245.6 ±31.9) mL/min?kg, (240.8 ±30.9)mL/min?kg, respectively, P<0.05].In the shunt group Qp/Qs was 2.16 ±0.38 and RVSP increased to (35.8 ±4.9) mmHg, RVHI was 0.3263 ± 0.0342, significantly higher than that of the other groups .The pulmonary arteriolar wall was evidently thickened in contrast to that in the sham group [ ( 22.3 ±1.7 )% vs.( 10.6 ±1.7 )%, P <0.05 ) .Conclusions Anastomosis of the left common carotid artery to left external jugular vein can successfully establish pulmonary arterial hypertension model induced by high blood flow in rats .
