Exploration on the selection of positive materials in active systemic anaphylaxis in guinea pigs
10.3969/j.issn.1671.7856.2015.009.010
- VernacularTitle:豚鼠主动全身过敏试验两种阳性物质选择的探讨
- Author:
Mei GAO
;
Chong CAO
;
Hui MA
;
Chunhua ZHU
- Publication Type:Journal Article
- Keywords:
Active systemic anaphylaxis;
Human albumin;
Ovalbumin;
Allergic reaction;
Guinea pigs
- From:
Chinese Journal of Comparative Medicine
2015;(9):51-55
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTheaimofthisstudywastoprovideabetterpositivecontrolforallergictestbycomparing the allergic effect of two kinds of positive materials , human albumin and ovalbumin , on active systemic anaphylaxis in guinea pig.Methods Guinea pigs were randomly divided into 14 groups, and were given human albumin , ovalbumin (2, 10, 100 mg/animal), or 0.9%sodium chloride injection as test substances , to assess the symptoms and incidence of systemic allergic responses induced by different sensitizing substances in different challenge doses and different challenge intervals.Results In the range of 2 to 100 mg/animal, the guinea pigs showed a 100%incidence rate of positive allergic reaction to human albumin and ovalbumin , the severity of anaphylactic symptoms was increasing along with the increase of sensitizing doses and challenge doses , and the allergic reaction was more strong induced by the same dose of ovalbumin than human albumin .Conclusions Our findings indicate that in the active systemic anaphylaxis test in guinea pigs , we recommend ovalbumin as the positive control in a dose of 2 mg/animal.
