Effects of Moxa Smoke Versus Tobacco Smoke on Autonomous Behaviors and Hippocampal GFAP Expression in Apolipoprotein E Gene Knockout Mice
10.13460/j.issn.1005-0957.2015.10.1009
- VernacularTitle:艾烟与香烟对载脂蛋白E基因敲除小鼠自主行为与海马GFAP表达的影响
- Author:
Juntian LIU
;
Yingxue CUI
;
Chang HUANG
;
Jian HUANG
;
Baixiao ZHAO
;
Li HAN
;
Jia YANG
;
Lei WANG
- Publication Type:Journal Article
- Keywords:
Moxibustion;
Moxa smoke;
Tobacco smoke;
ApoE-/-mice;
GFAP;
Autonomous behaviors
- From:
Shanghai Journal of Acupuncture and Moxibustion
2015;(10):1009-1012
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of moxa smoke versus tobaccosmoke on autonomous behaviors and hippocampal expression of glial fibrillary acidic protein (GFAP) in apolipoprotein E gene knockout (ApoE-/-) mice.Method Thirteen 8-week-old C57BL/6 mice constituted a blank control group. Twenty-seven ApoE-/-mice of the same age were randomized into ApoE-/-model, moxa smoke and tobacco smoke groups. The tobacco smoke and moxa smoke groups of mice were exposed to smoke 5-15 mg/m3circumstances. Every group of mice was intervened in 20 min. daily, six days a week, for atotal of 12 weeks. A behavioral test was conducted in week 13. The animals were then sacrificed to take the materials. Hippocampal GFAP in the brain was measured by an immunohistochemical method.ResultAutonomous activities were significantly more in theblank group than in the model group (P<0.05) and significantly fewer in the tobacco smoke group than in the blank group (P<0.05). There was no statistically significant difference in autonomous activities between the tobacco smoke group and the model or moxa smoke group (P>0.05). Moving distance was longer in the moxa smoke group than in the model group (P<0.05). Standing-up number was smaller in the moxa smoke group than in the blank group (P<0.05). Integral optical density of GFAP immune reaction products in the hippocampus was significantlyhigher in the model group of mice than in the blank and moxa smoke groups (P<0.05). Hippocampal GFAP expression was significantly higher in the group of mice than in the moxa smoke and blank groups (P<0.05).Conclusion Moxa smoke can increase the excitability of central nervous system in mice and reduce hippocampal GFAP expression in a mice model of Alzheimer disease.
