Clinical significance of enzyme linked immunosorbent assay and indirect immunofluorescence for detection of antinuclear antibody
10.3969/j.issn.1673-4130.2015.19.017
- VernacularTitle:比较酶联免疫吸附法与间接免疫荧光法检测抗核抗体的价值
- Author:
Guangjie WANG
;
Jie CHEN
;
Shijun LI
- Publication Type:Journal Article
- Keywords:
antinuclear antibody;
enzyme-linked immunosorbent method;
indirect immunofluorescence
- From:
International Journal of Laboratory Medicine
2015;(19):2814-2816
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the value of ELISA and indirect immunofluorescence (IIF)method in diagnosis for autoim-mune diseases.Methods A total of 33 patients in systemic lupus erythematosus(SLE)group,59 patients in other autoimmune dis-eases group,43 patients in non-autoimmune disease group,20 people accepted physical examination in control group.The antinuclear antibody (ANA)in each group were detected by two methods and analyzed.Results The high titer ANA detected by ELISA and IIF in SLE group and other autoimmune diseases group were (2.621±1.700),(2.248±1.781);(2.71 5±0.730),(2.544±0.59). The titer ANA detected by ELISA in non-autoimmune disease group was (1.034±1.050),which was lower than(2.253 ±0.691) detected by IIF.Conclusion ELSA might improve the detection effects of ANA antibody.
