Research on anti T47 D breast cancer activity and its G protein-coupled estrogen receptor pathway of tanshinone IIA
10.3969/j.issn.1001-1978.2015.10.026
- VernacularTitle:丹参酮IIA抗乳腺癌T47 D细胞增殖的GPER途径研究
- Author:
Piwen ZHAO
;
Jinfeng ZANG
;
Shiying TAO
;
Meng CHEN
;
Jianzhao NIU
- Publication Type:Journal Article
- Keywords:
tanshinone IIA;
breast cancer;
T47 D cell;
cell proliferation;
G protein-coupled estrogen re-ceptor;
estrogen receptor;
gene silence
- From:
Chinese Pharmacological Bulletin
2015;(10):1458-1462
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of tanshinone IIA on cell proliferation via G protein-coupled estrogen receptor inductive and regulative pathway in typical es-trogen receptor and G protein-coupled estrogen receptor positive T47D breast cancer cells. Methods The pro-liferation rate of T47 D cells influenced by tanshinone IIA was analyzed by MTT assay. G protein-coupled es-trogen receptor agonist G1 and GPER antagonist G15 were employed as tools. GPER SiRNA was applied to build GPER gene silence T47D cells. GPER expres-sion influenced by tanshinone IIA was measured by Western blot. Results The proliferation rates of T47D cells treated with 1 × 10 -5 mol·L-1 - 1 × 10 -7 mol· L-1 of tanshinone IIA were decreased significantly. Such effects could be attenuated by G1 or enhanced by G15 . Growth of GPER SiRNA transfected T47 D cells were significantly inhibited by 1 × 10 -5 mol·L-1 - 1 × 10 -7 mol·L-1 of tanshinone IIA treating. Result of Western blot showed that tanshinone IIA at 1 × 10 -5 mol· L-1 and 1 × 10 -6 mol · L-1 could induce de-crease of GPER protein expression in T47D cells. Conclusions Tanshinone IIA shows inhibitory effects on proliferation rate of T47 D breast cancer cells via GPER pathway. Tanshinone IIA could perform regula-tive function on GPER expression level in target cells.
