Inhibition of notoginsenoside R1 on SOCE in pulmonary arterial smooth muscle cells of pulmonary hypertension rats
10.3969/j.issn.1001-1978.2015.10.027
- VernacularTitle:三七皂苷R1对肺高压大鼠肺动脉平滑肌细胞SOCE的抑制作用
- Author:
Ruixing WANG
;
Mao DAI
;
Yunping MU
;
Jiao JIANG
;
Qiuhong HUANG
;
Zhijuan WU
;
Haixia JIAO
;
Mojun LIN
- Publication Type:Journal Article
- Keywords:
pulmonary hypertension;
chronic hypoxia;
monocrotaline;
notoginsenoside R1;
store-operated cal-cium entry;
pulmonary arterial smooth muscle cell
- From:
Chinese Pharmacological Bulletin
2015;(10):1463-1468
- CountryChina
- Language:Chinese
-
Abstract:
Aim To evaluate the effects of notoginsen-oside R1 on store-operated calcium entry ( SOCE ) in pulmonary arterial smooth muscle cells ( PASMCs ) of chronic hypoxia ( CH)-and monocrotaline ( MCT)-in-duced pulmonary hypertension ( PH) rats. Methods Mn2+ quenching of Fura-2 and measurement of intra-cellular free calcium concentration ( [ Ca2+] i ) using fluo-3 were examined in PASMCs of CH-exposed and MCT-treated rats. Results ①CH-exposed and MCT-treated rats exhibited profound PH when examined 3 weeks after hypoxia exposure or MCT injection, respec-tively. ②In the presence of 3 μmol·L-1 nifedipine, 10 μmol · L-1 notoginsenoside R1 significantly re-duced cyclopiazonic acid ( CPA )-induced the percent reduction in Fura-2 fluorescence measured 500 sec af-ter application of Mn2+, the maximal rate of Mn2+quenching, the amplitude of the Ca2+ influx transient and the resting [ Ca2+] i in PASMCs of CH-exposed and MCT-treated rats. Conclusion Notoginsenoside R1 inhibits SOCE and reduces resting [ Ca2+] i in PASMCs of CH-and MCT-induced PH rats.
