Techniques and improvements of establishing orthotopic liver transplantation rat models
10.3969/j.issn.2095-4344.2015.40.024
- VernacularTitle:原位肝移植模型的建立技巧与改进
- Author:
Jianhua BAI
;
Gang CHEN
;
Xinfeng ZHU
;
Fan WANG
;
Yingpeng ZHAO
;
Qiyu LIU
;
Li LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2015;(40):6526-6530
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:At present, liver transplantation is the only way to cure end-stage liver disease, but the complications after transplantation is stil an important factor of affecting the long-term survival of patients who received orthotopic liver transplantation, therefore it is necessary to establish a stable animal transplantation model. OBJECTIVE:To establish rat models of orthotopic liver transplantation. METHODS:After inhalation anesthesia with ether, 204 SD rats were perfused with 2-4℃ Ringer’s solution through the abdominal aorta. In order to reduce warm ischemia of the liver, the liver was not turned over before perfusion. The suprahepatic inferior vena cava was cut off along the phrenic ring after perfusion. No further trimming was needed when dressing, so as not to damage the vena cava. The donor liver was removed and preserved in 4℃Ringer’s liquid. The receptor liver was cut off and alogeneic orthotopic liver transplantation was performed using modified two-cuff method. After transplantation, rats could automaticaly turn over and drink water. Surviving more than 3 days is regarded as a successful transplantation. RESULTS AND CONCLUSION:102 liver transplantations were performed in 204 rats, with 86 rats surviving more than 3 days. The success rate of transplantation was 84%. The results demonstrate that rat models of orthotropic liver transplantation can be constructed successfuly through improving techniques.
