Determination of quetiapine in human hair by HPLC
- VernacularTitle:高效液相色谱法测定人头发中喹硫平浓度
- Author:
Yuechun ZHANG
;
Linlin SHI
;
Lize GU
;
Xujiang SU
;
Zhenlie LU
;
Weiming LING
- Publication Type:Journal Article
- Keywords:
hair;
quetiapine;
high performance liquid chromatography
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(9):161-163
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determinning quetiapine in human hair by HPLC.Methods A reverse phase HPLC system was performed on Inertsil ODS-C18 column (4.6 mm ×150 mm,5μm)with the mobile phase consisted of 0.01 mol/Lammonioum-methanol(32︰68).The flow rate was 1.0 mL/min,column temperature was 40℃,the detection wavelength was 254 nm.N-hexane was used as extracting solvent.Results The calibration curve was linear in the range of 5-200 ng/mg.for quetiapine.The recovery of extraction >75.0%, the recovery of method was between 95.0% and 105.0%, the intra-day and inter-day precision were all no more than 10.0%.This method met the requirements of biological samples. Conclusion A HPLC method of concentration of quetiapine in human hair is established, which is simple,sensitive,accurate and has a certain value.
