Effect of astragalus extracts on bone marrow-derived endothelial progenitor cells in rats
10.11958/j.issn.0253-9896.2015.10.002
- VernacularTitle:黄芪提取物对大鼠骨髓源性内皮祖细胞的作用
- Author:
Nuan LIU
;
Bingyu MAO
;
Lei YANG
;
Guochang XU
;
Songshan YE
;
Peihua ZHANG
;
Lifang ZHANG
- Publication Type:Journal Article
- Keywords:
Astragalus membranaceus;
myeloid progenitor cells;
cell adhesion;
cell movement;
endothelial progenitor cells;
angiogenesis;
endothelial nitric oxide synthase
- From:
Tianjin Medical Journal
2015;(10):1093-1096
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of astragalus extracts on adherence, migration, cell viability, microvascular formation, and eNOS expression in bone marrow-derived endothelial progenitor cells (EPCs) of rats. Methods EPCs were cultured, isolated and identified in vitro and divided into four groups: low titer group (10-4 g/L of astragalus extracts), middle titer group (10-3 g/L of astragalus extracts), high titer group (10-2 g/L of astragalus extracts) and control group. The effects of astragalus extract on adhesion, migration or microvascular formation were observed under the inverted microscope and com?pared between all groups;Cell viability was detected by MTT assay;mRNA transcription and protein expression levels of en?dothelial nitric oxide synthase (eNOS) in EPCs were detected by reverse transcription PCR (RT-PCR) and Western blot re?spectively. Results Compared with the control group, cell adhesion, migration and microvascular formation of EPCs all en?hanced with addition of astragalus extracts in a dose dependent manner (F=15.256, 13.633, 97.549 respectively, and P <0.05 in all cases); Cell vitality of EPCs increased with administration of astragalus extracts in a time and dose dependant manner. (F=9.755 for time and F=10.18 for dose). mRNA transcription and protein expression of eNOS in EPCs were up-reg?ulated with addition of astragalus extracts in a dose dependent manner (F=56.356 and 77.125 respectively, and P<0.05 in both cases). Conclusion Astragalus extracts play important role in angiogenesis in EPCs probably through up-regulating expressions of eNOS.
