The effect of up-regulating miR-34c-5p on the proliferation and invasion of nasopharyngeal carcinoma cell line HONE-1
10.11958/j.issn.0253-9896.2015.10.003
- VernacularTitle:上调miR-34c-5p对鼻咽癌细胞HONE-1增殖和侵袭的影响
- Author:
Feifei MA
;
Yanqiang HAO
;
Guoqiang WANG
;
Xin ZHANG
;
Cheng ZHI
;
Tianxing JI
- Publication Type:Journal Article
- Keywords:
nasopharyngeal neoplasms;
cell proliferation;
neoplasm invasiveness;
in vitro;
miR-34c-5p;
HONE-1
- From:
Tianjin Medical Journal
2015;(10):1097-1099
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of up-regulating miR-34c-5p on cloning formation and migration of nasopharyngeal carcinoma cell line HONE-1 in vitro. Methods Expression levels of miR-34c-5p in nasopharyngeal cell line NP69 and nasopharyngeal carcinoma cell line HONE-1 were investigated by real time quantitative PCR;Cloning Forma?tion Test and Transwell Migration Assay were used to explore the effect of up-regulating miR-34c-5p on proliferation and migration of nasopharyngeal carcinoma cell line HONE-1. Results The expression of miR-34c-5p in nasopharyngeal car?cinoma cell line HONE-1 was lower than that in normal nasopharyngeal cell line NP69. Up-regulating miR-34c-5p signifi?cantly suppressed the cloning formation and migration capacity, compared to those of NP69 cell line and HONE-1 with nor?mal level of miR-34c-5p(P<0.05). Conclusion Down-regulating miR-34c-5p involve in proliferation and migration of nasopharyngeal carcinoma and may be a used as a new therapeutic target for nasopharyngeal carcinoma.
